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KMID : 0368019830060040383
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Journal of Soonchunhyang University
1983 Volume.6 No. 4 p.383 ~ p.388
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A Scanning Electron Microscopic Study on the Epidermal Surface of Aging Frog(Xenopus laevis)
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Abstract
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Surface structure of the abdominal skins of young(one year old) and aged(five and a half years oldt frogs, Xenopus laevs, have been observed by scanning electron microscopy. rPieces of the skin were taken from. the central area of the abdomen; fixed in Karnovsky´s fixative (phosphate buffer, PI-17.3) and post-fixed in 1% osmium tetroxide(phosphate buffer, pH 7.3). Following fixation, the tissues were dehydrated in graded concentrations of ethanol and amyl acetate(1 : 1), and were followed by gradual increase in the volume of am; 1 acetate until a total substitution(100% amyl acetate) was achieved. Amyl acetate in the tissues were replaced with liquid COz; and dried in a "Polaron" critical point dryer. Blocks of tissues were
oriented mounted and attachedby silver conducting paint on specimen stubs. All specimens were coated with gold in a vaccum evaporator. The samples were studied in a ISM-U3 scinr.I
electron mieroscpe operated at an acceleration voltage of 15 KV.
Observed results were as follow;
1. Based on the surface morphology two types of epidermal cells(Type and Type ;i ) are recognized:
2. Type l cells are smooth-surfaced and more numerous than type II cells that leave irregular surface terrains m hich appear to result from collections of mucoid substances over areas of crowded microridges.
3. Both type of epithelial cells have numerous microridges of 100?110 nm throughout the surface in young frogs.
4. The microridges in aged frogs are more numerous and wider(160-u170 nm) than in young ones, and tend to produce variously,., modified appearance in aged frogs.
5. Along the periphery of epithelial cells are discrete marginal microridges which in young frogs are bridged by interconnecting cross-links.
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